Predicted Highly eXpressed and Putative Alien genes

• If your genome has more than one chromosome or megaplasmid, go here.

• For a brief explanation how to use this program and how to read the output files, go here.

• To learn more about PHX and PA genes check some relevant references.

• Known bugs

• Note: this is a free service provided without any warranty explicit or implied.

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Step 1: upload the sequence and annotations in GenBank format (example):

(for complete prokaryotic genomes download .gbk files here)

IMPORTANT: the exact format of the input files is essential. In particular, the 'CDS' lines have to include exactly five leading spaces followed by CDS and 13 additional spaces.

 

Step 2: upload ribosomal protein annotations (example):

Step 3: upload chaperone annotations (example):

Step 4: upload translation/transcription factor annotations (example):

Step 5: choose genetic code:

 

Standard Mycoplasma (TGA translates as Trp)

 

Step 6: choose format for graphic output:

 

pdf ps (PostScript)

 

Step 7: enter the EMAIL address where you would like to receive the results:

 

 

Step 8: click Submit to complete your request:

 

Note: please click Submit button just ONCE. It may take several minutes to process your request.